parental pc9 cells Search Results


retroviral transduction pc9 parental cells  (ATCC)
99
ATCC retroviral transduction pc9 parental cells
Retroviral Transduction Pc9 Parental Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parental+pc9+cells/pm29909007-28-8-53?v=ATCC
Average 99 stars, based on 1 article reviews
retroviral transduction pc9 parental cells - by Bioz Stars, 2026-08
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parental pc 9 cells  (Selleck Chemicals)
96
Selleck Chemicals parental pc 9 cells
Parental Pc 9 Cells, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parental+pc9+cells/pm36182943-138-6-18?v=Selleck+Chemicals
Average 96 stars, based on 1 article reviews
parental pc 9 cells - by Bioz Stars, 2026-08
96/100 stars
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parental nsclc cell lines  (ATCC)
99
ATCC parental nsclc cell lines
CircSOX13 is highly expressed in <t>NSCLC</t> . A. RT-qPCR to detect circSOX13 expression in NSCLC tissues and normal tissues adjacent to cancer; B. RT-qPCR to detect circSOX13 expression in serum of normal subjects and patients with NSCLC; C. RT-qPCR to detectcircSOX13 expression in <t>PC9,</t> SPC-A1, <t>H1975,</t> <t>H1299</t> and <t>A549</t> <t>and</t> <t>16HBE;</t> the values were expressed by mean ± SD (C, n = 3); versus the normal tissue group, * P < 0.05; versus the 16HBE group,^ P < 0.05.
Parental Nsclc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parental+pc9+cells/pmc08805859-50-8-21?v=ATCC
Average 99 stars, based on 1 article reviews
parental nsclc cell lines - by Bioz Stars, 2026-08
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luad cell lines  (Procell Inc)
86
Procell Inc luad cell lines
Bioinformatic analysis <t>of</t> <t>DDP</t> resistance–related genes in <t>LUAD.</t> (A) Volcano plot depicting DEGs in DDP‐resistant cells. (B) Venn diagram illustrating the intersection between genes from the GSE157692 dataset and DDP resistance–related targets, identifying 345 overlapping core genes. (C) KEGG pathway enrichment analysis of the core genes. (D–F) GO enrichment analysis for BP, CC, and MF.
Luad Cell Lines, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/parental+pc9+cells/pmc13195626-26-2-19?v=Procell+Inc
Average 86 stars, based on 1 article reviews
luad cell lines - by Bioz Stars, 2026-08
86/100 stars
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Image Search Results


CircSOX13 is highly expressed in NSCLC . A. RT-qPCR to detect circSOX13 expression in NSCLC tissues and normal tissues adjacent to cancer; B. RT-qPCR to detect circSOX13 expression in serum of normal subjects and patients with NSCLC; C. RT-qPCR to detectcircSOX13 expression in PC9, SPC-A1, H1975, H1299 and A549 and 16HBE; the values were expressed by mean ± SD (C, n = 3); versus the normal tissue group, * P < 0.05; versus the 16HBE group,^ P < 0.05.

Journal: Bioengineered

Article Title: Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1

doi: 10.1080/21655979.2021.1997223

Figure Lengend Snippet: CircSOX13 is highly expressed in NSCLC . A. RT-qPCR to detect circSOX13 expression in NSCLC tissues and normal tissues adjacent to cancer; B. RT-qPCR to detect circSOX13 expression in serum of normal subjects and patients with NSCLC; C. RT-qPCR to detectcircSOX13 expression in PC9, SPC-A1, H1975, H1299 and A549 and 16HBE; the values were expressed by mean ± SD (C, n = 3); versus the normal tissue group, * P < 0.05; versus the 16HBE group,^ P < 0.05.

Article Snippet: Normal human bronchial epithelial cell line (16HBE) and parental NSCLC cell lines (PC9, SPC-A1, H1975, H1299 and A549) were bought from ATCC and placed in incubators under at 37°C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Expressing

Knocking down circSOX13 inhibits NSCLC proliferation, migration and invasion but promotes apoptosis . After transfecting circSOX13-shRNA, A. RT-qPCR to detect circSOX13 expression in A549 and H1299; B. CCK-8 to detect the viability of A549 and H1299; C. Edu analysis to detect A549 and H1299 proliferation cell; D. Flow cytometry to detect A549 and H1299 apoptosis rate; E. Transwell to detect A549 and H1299 migration and invasion capabilities; the values were expressed by mean ± SD (n = 3); versus sh-NC group, * P < 0.05.

Journal: Bioengineered

Article Title: Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1

doi: 10.1080/21655979.2021.1997223

Figure Lengend Snippet: Knocking down circSOX13 inhibits NSCLC proliferation, migration and invasion but promotes apoptosis . After transfecting circSOX13-shRNA, A. RT-qPCR to detect circSOX13 expression in A549 and H1299; B. CCK-8 to detect the viability of A549 and H1299; C. Edu analysis to detect A549 and H1299 proliferation cell; D. Flow cytometry to detect A549 and H1299 apoptosis rate; E. Transwell to detect A549 and H1299 migration and invasion capabilities; the values were expressed by mean ± SD (n = 3); versus sh-NC group, * P < 0.05.

Article Snippet: Normal human bronchial epithelial cell line (16HBE) and parental NSCLC cell lines (PC9, SPC-A1, H1975, H1299 and A549) were bought from ATCC and placed in incubators under at 37°C with 5% CO 2 .

Techniques: Migration, shRNA, Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry

CircSOX13 acts as a sponge for miR-3194-3p . A. Via bioinformatics website http://starbase.sysu.edu.cn/ to query the potential binding sites of miR-3194-3p and circSOX13; B. RT-qPCR to detect miR-3194-3p in NSCLC tissues and normal tissue adjacent to cancer; C. RT-qPCR to detect miR-3194-3p expression in PC9, SPC-A1, H1975, H1299 and A549 and 16HBE; D. RT-qPCR to detect miR-3194-3p expression in A549 and H1299 after transfecting circSOX13-shRNA; E. DLR experiment to detect the targeted binding relationship between miR-3194-3p and circSOX13; the values were mean ± SD (n = 3); versus the normal tissue group, * P < 0.05; versus the 16HBE group, ^ P < 0.05; versus the sh-NC group, # P < 0.05; versus the mimic NC group, & P < 0.05.

Journal: Bioengineered

Article Title: Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1

doi: 10.1080/21655979.2021.1997223

Figure Lengend Snippet: CircSOX13 acts as a sponge for miR-3194-3p . A. Via bioinformatics website http://starbase.sysu.edu.cn/ to query the potential binding sites of miR-3194-3p and circSOX13; B. RT-qPCR to detect miR-3194-3p in NSCLC tissues and normal tissue adjacent to cancer; C. RT-qPCR to detect miR-3194-3p expression in PC9, SPC-A1, H1975, H1299 and A549 and 16HBE; D. RT-qPCR to detect miR-3194-3p expression in A549 and H1299 after transfecting circSOX13-shRNA; E. DLR experiment to detect the targeted binding relationship between miR-3194-3p and circSOX13; the values were mean ± SD (n = 3); versus the normal tissue group, * P < 0.05; versus the 16HBE group, ^ P < 0.05; versus the sh-NC group, # P < 0.05; versus the mimic NC group, & P < 0.05.

Article Snippet: Normal human bronchial epithelial cell line (16HBE) and parental NSCLC cell lines (PC9, SPC-A1, H1975, H1299 and A549) were bought from ATCC and placed in incubators under at 37°C with 5% CO 2 .

Techniques: Binding Assay, Quantitative RT-PCR, Expressing, shRNA

CircSOX13 influences malignant behavior of NSCLC by regulating miR-3194-3p . After transfecting miR-3194-3p mimic, A. RT-qPCR to detect miR-3194-3p expression in A549 and H1299 cells; B. CCK-8 to detect A549 and H1299 cell viability; C. Edu analysis to detect A549 and H1299 proliferation; D. Flow cytometry to detect A549 and H1299 apoptosis rate; E. Transwell to detect A549 and H1299 invasion and migration ability; the values were expressed as mean ± SD (n = 3); versus the mimic NC group, * P < 0.05.

Journal: Bioengineered

Article Title: Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1

doi: 10.1080/21655979.2021.1997223

Figure Lengend Snippet: CircSOX13 influences malignant behavior of NSCLC by regulating miR-3194-3p . After transfecting miR-3194-3p mimic, A. RT-qPCR to detect miR-3194-3p expression in A549 and H1299 cells; B. CCK-8 to detect A549 and H1299 cell viability; C. Edu analysis to detect A549 and H1299 proliferation; D. Flow cytometry to detect A549 and H1299 apoptosis rate; E. Transwell to detect A549 and H1299 invasion and migration ability; the values were expressed as mean ± SD (n = 3); versus the mimic NC group, * P < 0.05.

Article Snippet: Normal human bronchial epithelial cell line (16HBE) and parental NSCLC cell lines (PC9, SPC-A1, H1975, H1299 and A549) were bought from ATCC and placed in incubators under at 37°C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry, Migration

MAPRE1 is the target gene of miR-3194-3p . A. RT-qPCR to detect MAPRI1 expression in NSCLC tissues and normal tissues adjacent to cancer; B. Western blot to detect MAPRI1 expression in PC9, SPC-A1, H1975, H1299 and A549 and 16HBE C. Western blot to detect MAPRE1 expression in A549 and H1299 cells after transfecting miR-3194-3p mimic; D. Via bioinformatics website http://starbase.sysu.edu.cn/ to query the potential binding sites of miR-3194-3p and MAPRE1; E. DLR experiment to check the targeting relationship between miR-3194-3p and MAPRE1; the values were expressed as mean ± SD (n = 3); versus the normal tissue group, * P < 0.05; versus the 16HBE group, ^ P < 0.05; versus the mimic NC group, # P < 0.05.

Journal: Bioengineered

Article Title: Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1

doi: 10.1080/21655979.2021.1997223

Figure Lengend Snippet: MAPRE1 is the target gene of miR-3194-3p . A. RT-qPCR to detect MAPRI1 expression in NSCLC tissues and normal tissues adjacent to cancer; B. Western blot to detect MAPRI1 expression in PC9, SPC-A1, H1975, H1299 and A549 and 16HBE C. Western blot to detect MAPRE1 expression in A549 and H1299 cells after transfecting miR-3194-3p mimic; D. Via bioinformatics website http://starbase.sysu.edu.cn/ to query the potential binding sites of miR-3194-3p and MAPRE1; E. DLR experiment to check the targeting relationship between miR-3194-3p and MAPRE1; the values were expressed as mean ± SD (n = 3); versus the normal tissue group, * P < 0.05; versus the 16HBE group, ^ P < 0.05; versus the mimic NC group, # P < 0.05.

Article Snippet: Normal human bronchial epithelial cell line (16HBE) and parental NSCLC cell lines (PC9, SPC-A1, H1975, H1299 and A549) were bought from ATCC and placed in incubators under at 37°C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Binding Assay

CircSOX13 promotes the malignant behavior of NSCLC via miR-3194-3p/MAPRE1 axis . After transfecting oe-SOX13 and si-MAPRE1, A. RT-qPCR or western blot to detect circSOX13, miR-3194-3p and MAPRI1 expression; B. CCK-8 to detect A549 and H1299 viability; C. Edu analysis to detect A549 and H1299 proliferation; D. Flow cytometry to detect A549 and H1299 apoptosis rate; E. Transwell to detect A549 and H1299 invasion and migration abilities; the values were expressed as mean ± SD (n = 3); versus the oe-NC group, * P < 0.05; versus the oe-SOX13 + si-NC group, ^ P < 0.05.

Journal: Bioengineered

Article Title: Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1

doi: 10.1080/21655979.2021.1997223

Figure Lengend Snippet: CircSOX13 promotes the malignant behavior of NSCLC via miR-3194-3p/MAPRE1 axis . After transfecting oe-SOX13 and si-MAPRE1, A. RT-qPCR or western blot to detect circSOX13, miR-3194-3p and MAPRI1 expression; B. CCK-8 to detect A549 and H1299 viability; C. Edu analysis to detect A549 and H1299 proliferation; D. Flow cytometry to detect A549 and H1299 apoptosis rate; E. Transwell to detect A549 and H1299 invasion and migration abilities; the values were expressed as mean ± SD (n = 3); versus the oe-NC group, * P < 0.05; versus the oe-SOX13 + si-NC group, ^ P < 0.05.

Article Snippet: Normal human bronchial epithelial cell line (16HBE) and parental NSCLC cell lines (PC9, SPC-A1, H1975, H1299 and A549) were bought from ATCC and placed in incubators under at 37°C with 5% CO 2 .

Techniques: Quantitative RT-PCR, Western Blot, Expressing, CCK-8 Assay, Flow Cytometry, Migration

CircSOX13accelerates NSCLC cisplatin resistance . A. CCK-8 to detect the IC50 value of A549 and H1299 as well as A549/DDP and H1299/DDP; B. RT-qPCR or western blot to detect circSOX13, MAPRE1 and miR-3194-3p expression in A549 and H1299 and A549/DDP and H1299/DDP; After transfecting circSOX13-shRNA, C. RT-qPCR to detect circSOX13 expression in A549/DDP and H1299/DDP; D. CCK-8 to detect IC 50 value in A549/DDP and H1299/DDP; E. DNA replication of A549/DDP and H1299/DDP cells detected by Edu analysis; F. Flow cytometry to detect the apoptosis of A549/DDP and H1299/DDP; G. Transwell to detect A549/DDP and H1299/DDP invasion and migration abilities; the values were expressed by mean ± SD (n = 3); versus the A549 group or the sh-NC group, * P < 0.05.

Journal: Bioengineered

Article Title: Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1

doi: 10.1080/21655979.2021.1997223

Figure Lengend Snippet: CircSOX13accelerates NSCLC cisplatin resistance . A. CCK-8 to detect the IC50 value of A549 and H1299 as well as A549/DDP and H1299/DDP; B. RT-qPCR or western blot to detect circSOX13, MAPRE1 and miR-3194-3p expression in A549 and H1299 and A549/DDP and H1299/DDP; After transfecting circSOX13-shRNA, C. RT-qPCR to detect circSOX13 expression in A549/DDP and H1299/DDP; D. CCK-8 to detect IC 50 value in A549/DDP and H1299/DDP; E. DNA replication of A549/DDP and H1299/DDP cells detected by Edu analysis; F. Flow cytometry to detect the apoptosis of A549/DDP and H1299/DDP; G. Transwell to detect A549/DDP and H1299/DDP invasion and migration abilities; the values were expressed by mean ± SD (n = 3); versus the A549 group or the sh-NC group, * P < 0.05.

Article Snippet: Normal human bronchial epithelial cell line (16HBE) and parental NSCLC cell lines (PC9, SPC-A1, H1975, H1299 and A549) were bought from ATCC and placed in incubators under at 37°C with 5% CO 2 .

Techniques: CCK-8 Assay, Quantitative RT-PCR, Western Blot, Expressing, shRNA, Flow Cytometry, Migration

CircSOX13 promotes cisplatin resistance in NSCLC via miR-3194-3p /MAPRE1 axis . A. The expression of MAPRE1 and miR-3194-3p in A549/DDP and H1299/DDP cells after co-transfection oe-SOX13 and miR-3194-3p mimic detected by RT-qPCR or Western blot; B. IC 50 values of A549/DDP and H1299/DDP cells after co-transfection oe-circSOX13 and miR-3194-3p mimic detected by CCK-8; C. DNA replication in A549/DDP and H1299/DDP cells after co-transfection with oe-circSOX13 and miR-3194-3p mimic detected by Edu; D. Apoptosis of A549/DDP and H1299/DDP cells after co-transfection with oe-circSOX13 and miR-3194-3p mimic detected by flow cytometry; E. Transwell to detect the invasion and migration abilities of A549/DDP and H1299/DDP cells after co-transfection with oe-circSOX13 and miR-3194-3p mimic. The values were expressed as mean ± SD (n = 3); versus the oe-NC group, * P < 0.05; versus the oe-SOX13 + mimic NC group, ^ P < 0.05.

Journal: Bioengineered

Article Title: Circular RNA SOX13 promotes malignant behavior and cisplatin resistance in non-small cell lung cancer through targeting microRNA-3194-3p/microtubule-associated protein RP/EB family member 1

doi: 10.1080/21655979.2021.1997223

Figure Lengend Snippet: CircSOX13 promotes cisplatin resistance in NSCLC via miR-3194-3p /MAPRE1 axis . A. The expression of MAPRE1 and miR-3194-3p in A549/DDP and H1299/DDP cells after co-transfection oe-SOX13 and miR-3194-3p mimic detected by RT-qPCR or Western blot; B. IC 50 values of A549/DDP and H1299/DDP cells after co-transfection oe-circSOX13 and miR-3194-3p mimic detected by CCK-8; C. DNA replication in A549/DDP and H1299/DDP cells after co-transfection with oe-circSOX13 and miR-3194-3p mimic detected by Edu; D. Apoptosis of A549/DDP and H1299/DDP cells after co-transfection with oe-circSOX13 and miR-3194-3p mimic detected by flow cytometry; E. Transwell to detect the invasion and migration abilities of A549/DDP and H1299/DDP cells after co-transfection with oe-circSOX13 and miR-3194-3p mimic. The values were expressed as mean ± SD (n = 3); versus the oe-NC group, * P < 0.05; versus the oe-SOX13 + mimic NC group, ^ P < 0.05.

Article Snippet: Normal human bronchial epithelial cell line (16HBE) and parental NSCLC cell lines (PC9, SPC-A1, H1975, H1299 and A549) were bought from ATCC and placed in incubators under at 37°C with 5% CO 2 .

Techniques: Expressing, Cotransfection, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Flow Cytometry, Migration

Bioinformatic analysis of DDP resistance–related genes in LUAD. (A) Volcano plot depicting DEGs in DDP‐resistant cells. (B) Venn diagram illustrating the intersection between genes from the GSE157692 dataset and DDP resistance–related targets, identifying 345 overlapping core genes. (C) KEGG pathway enrichment analysis of the core genes. (D–F) GO enrichment analysis for BP, CC, and MF.

Journal: International Journal of Genomics

Article Title: USP44 Stabilizes MAOB via Deubiquitination to Inhibit Cisplatin Resistance in Lung Adenocarcinoma

doi: 10.1155/ijog/7433804

Figure Lengend Snippet: Bioinformatic analysis of DDP resistance–related genes in LUAD. (A) Volcano plot depicting DEGs in DDP‐resistant cells. (B) Venn diagram illustrating the intersection between genes from the GSE157692 dataset and DDP resistance–related targets, identifying 345 overlapping core genes. (C) KEGG pathway enrichment analysis of the core genes. (D–F) GO enrichment analysis for BP, CC, and MF.

Article Snippet: The parental LUAD cell lines (A549 and PC9) and their corresponding DDP‐resistant derivatives (A549‐DDP and PC9‐DDP) were obtained from Procell Biotechnology Co., Ltd. (Wuhan, China).

Techniques:

Expression and prognostic analysis of MAOB in LUAD and DDP‐resistant cell lines. (A) MAOB expression in A549 and A549‐DDP cells was analyzed using the GSE157692 dataset. (B) MAOB expression in LUAD ( n = 483) and normal tissues ( n = 59) was assessed via GEPIA. (C) Transcript levels of MAOB in normal ( n = 59) and primary LUAD tumor ( n = 515) samples from TCGA were compared. (D) Kaplan–Meier survival analysis of LUAD patients stratified by MAOB expression level ( p = 0.0047). (E, F) MAOB protein levels in A549/A549‐DDP and PC9/PC9‐DDP cells were detected by Western blot. ∗ p < 0.05; ∗∗ p < 0.01.

Journal: International Journal of Genomics

Article Title: USP44 Stabilizes MAOB via Deubiquitination to Inhibit Cisplatin Resistance in Lung Adenocarcinoma

doi: 10.1155/ijog/7433804

Figure Lengend Snippet: Expression and prognostic analysis of MAOB in LUAD and DDP‐resistant cell lines. (A) MAOB expression in A549 and A549‐DDP cells was analyzed using the GSE157692 dataset. (B) MAOB expression in LUAD ( n = 483) and normal tissues ( n = 59) was assessed via GEPIA. (C) Transcript levels of MAOB in normal ( n = 59) and primary LUAD tumor ( n = 515) samples from TCGA were compared. (D) Kaplan–Meier survival analysis of LUAD patients stratified by MAOB expression level ( p = 0.0047). (E, F) MAOB protein levels in A549/A549‐DDP and PC9/PC9‐DDP cells were detected by Western blot. ∗ p < 0.05; ∗∗ p < 0.01.

Article Snippet: The parental LUAD cell lines (A549 and PC9) and their corresponding DDP‐resistant derivatives (A549‐DDP and PC9‐DDP) were obtained from Procell Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot

MAOB overexpression enhances DDP sensitivity in DDP‐resistant LUAD cells by inhibiting proliferation and promoting apoptosis. (A) MAOB overexpression efficiency was validated in A549‐DDP and PC9‐DDP cells via Western blot. (B, C) CCK‐8 assays were performed to determine DDP sensitivity in A549‐DDP and PC9‐DDP cells. (D–G) A549‐DDP and PC9‐DDP cells were transfected with pcDNA or MAOB‐overexpressing plasmid and divided into two subgroups: control (without DDP treatment) and DDP treatment. (D, E) Colony formation assays were conducted to assess cell proliferation in A549‐DDP and PC9‐DDP cells. (F, G) Flow cytometry analysis was used to detect cell apoptosis. Data are presented as mean ± SD from n ≥ 3 independent biological replicates. ∗ p < 0.05; ∗∗ p < 0.01.

Journal: International Journal of Genomics

Article Title: USP44 Stabilizes MAOB via Deubiquitination to Inhibit Cisplatin Resistance in Lung Adenocarcinoma

doi: 10.1155/ijog/7433804

Figure Lengend Snippet: MAOB overexpression enhances DDP sensitivity in DDP‐resistant LUAD cells by inhibiting proliferation and promoting apoptosis. (A) MAOB overexpression efficiency was validated in A549‐DDP and PC9‐DDP cells via Western blot. (B, C) CCK‐8 assays were performed to determine DDP sensitivity in A549‐DDP and PC9‐DDP cells. (D–G) A549‐DDP and PC9‐DDP cells were transfected with pcDNA or MAOB‐overexpressing plasmid and divided into two subgroups: control (without DDP treatment) and DDP treatment. (D, E) Colony formation assays were conducted to assess cell proliferation in A549‐DDP and PC9‐DDP cells. (F, G) Flow cytometry analysis was used to detect cell apoptosis. Data are presented as mean ± SD from n ≥ 3 independent biological replicates. ∗ p < 0.05; ∗∗ p < 0.01.

Article Snippet: The parental LUAD cell lines (A549 and PC9) and their corresponding DDP‐resistant derivatives (A549‐DDP and PC9‐DDP) were obtained from Procell Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Over Expression, Western Blot, CCK-8 Assay, Transfection, Plasmid Preparation, Control, Flow Cytometry

MAOB overexpression enhances DDP‐mediated inhibition of migration, invasion, and EMT in DDP‐resistant LUAD cells. Untreated or DDP‐treated A549‐DDP and PC9‐DDP cells were transfected with pcDNA or MAOB. (A–D) Transwell migration assay was conducted to assess cell migration and invasion. (E, F) Western blot analysis of EMT marker (E‐cadherin, N‐cadherin, and vimentin) expression levels. Data are presented as mean ± SD from n ≥ 3 independent biological replicates. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Journal: International Journal of Genomics

Article Title: USP44 Stabilizes MAOB via Deubiquitination to Inhibit Cisplatin Resistance in Lung Adenocarcinoma

doi: 10.1155/ijog/7433804

Figure Lengend Snippet: MAOB overexpression enhances DDP‐mediated inhibition of migration, invasion, and EMT in DDP‐resistant LUAD cells. Untreated or DDP‐treated A549‐DDP and PC9‐DDP cells were transfected with pcDNA or MAOB. (A–D) Transwell migration assay was conducted to assess cell migration and invasion. (E, F) Western blot analysis of EMT marker (E‐cadherin, N‐cadherin, and vimentin) expression levels. Data are presented as mean ± SD from n ≥ 3 independent biological replicates. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: The parental LUAD cell lines (A549 and PC9) and their corresponding DDP‐resistant derivatives (A549‐DDP and PC9‐DDP) were obtained from Procell Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Over Expression, Inhibition, Migration, Transfection, Transwell Migration Assay, Western Blot, Marker, Expressing

USP44 stabilizes MAOB via deubiquitination in DDP‐resistant LUAD cells. (A) USP44 expression in A549 and A549‐DDP cells was analyzed using the GSE157692 dataset. (B) Transcript levels of USP44 in normal ( n = 59) and primary LUAD tumor ( n = 515) samples from TCGA were compared. (C) Kaplan–Meier survival analysis of LUAD patients stratified by USP44 expression level ( p = 0.044). (D, E) Co‐IP assays in A549‐DDP and PC9‐DDP cells showed that USP44 overexpression decreased MAOB ubiquitination. (F, G) CHX chase assay was employed to assess MAOB protein stability in A549‐DDP and PC9‐DDP cells transfected with pcDNA or USP44. Data are presented as mean ± SD from n ≥ 3 independent biological replicates. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Journal: International Journal of Genomics

Article Title: USP44 Stabilizes MAOB via Deubiquitination to Inhibit Cisplatin Resistance in Lung Adenocarcinoma

doi: 10.1155/ijog/7433804

Figure Lengend Snippet: USP44 stabilizes MAOB via deubiquitination in DDP‐resistant LUAD cells. (A) USP44 expression in A549 and A549‐DDP cells was analyzed using the GSE157692 dataset. (B) Transcript levels of USP44 in normal ( n = 59) and primary LUAD tumor ( n = 515) samples from TCGA were compared. (C) Kaplan–Meier survival analysis of LUAD patients stratified by USP44 expression level ( p = 0.044). (D, E) Co‐IP assays in A549‐DDP and PC9‐DDP cells showed that USP44 overexpression decreased MAOB ubiquitination. (F, G) CHX chase assay was employed to assess MAOB protein stability in A549‐DDP and PC9‐DDP cells transfected with pcDNA or USP44. Data are presented as mean ± SD from n ≥ 3 independent biological replicates. ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: The parental LUAD cell lines (A549 and PC9) and their corresponding DDP‐resistant derivatives (A549‐DDP and PC9‐DDP) were obtained from Procell Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Co-Immunoprecipitation Assay, Over Expression, Ubiquitin Proteomics, Transfection

MAOB knockdown reverses the effects of USP44 overexpression on DDP resistance, proliferation, apoptosis, migration, invasion, and EMT in LUAD cells. A549‐DDP and PC9‐DDP cells were divided into three groups: pcDNA + siNC, USP44 + siNC, and USP44 + siMAOB. (A, B) Western blot was used to validate the efficiency of MAOB knockdown in USP44‐overexpressing A549‐DDP and PC9‐DDP cells. (C) Colony formation assay was used to assess cell proliferation. (D) Flow cytometry analysis of cell apoptosis. (E, F) Transwell assay was performed to evaluate cell migration and invasion. (G, H) Western blot analysis of E‐cadherin, N‐cadherin, and vimentin expression. Data are presented as mean ± SD from n ≥ 3 independent biological replicates. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Journal: International Journal of Genomics

Article Title: USP44 Stabilizes MAOB via Deubiquitination to Inhibit Cisplatin Resistance in Lung Adenocarcinoma

doi: 10.1155/ijog/7433804

Figure Lengend Snippet: MAOB knockdown reverses the effects of USP44 overexpression on DDP resistance, proliferation, apoptosis, migration, invasion, and EMT in LUAD cells. A549‐DDP and PC9‐DDP cells were divided into three groups: pcDNA + siNC, USP44 + siNC, and USP44 + siMAOB. (A, B) Western blot was used to validate the efficiency of MAOB knockdown in USP44‐overexpressing A549‐DDP and PC9‐DDP cells. (C) Colony formation assay was used to assess cell proliferation. (D) Flow cytometry analysis of cell apoptosis. (E, F) Transwell assay was performed to evaluate cell migration and invasion. (G, H) Western blot analysis of E‐cadherin, N‐cadherin, and vimentin expression. Data are presented as mean ± SD from n ≥ 3 independent biological replicates. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: The parental LUAD cell lines (A549 and PC9) and their corresponding DDP‐resistant derivatives (A549‐DDP and PC9‐DDP) were obtained from Procell Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Knockdown, Over Expression, Migration, Western Blot, Colony Assay, Flow Cytometry, Transwell Assay, Expressing